Programme for training school

Monday 23rd November: Making Models

10.00 – 10.45 Introduction to the Aims of the Course
10.45 – 11.15 MouseAGE COST Action
11.30 – 12.30

Introduction to Modelling Disease in Mice
The utility of mouse models in understanding disease.
Key examples of advances resulting from mouse studies including limitations of the mouse and arguments against using it as a model.

14.00 - 15.00

Overview of Existing Technologies to Generate Mouse Models
Summary of current technologies and strains. Transgenics, Talens, alleles.

15.00 – 16.00

CRISPR/Cas 9 Technologies
Mix of short talks and worked examples of how to generate CRISPR mutations, problems, status of technologies and outputs. Including worked examples of use of software packages for the design of CRISPRs, cloning strategies, analysis of outputs from CRISPR projects.

Tuesday 24th November: Analysing Models

Session 1: Phenotyping

9.30 – 10.30

Phenotyping Platforms
Utility and outputs from common phenotyping platforms. Importance of comprehensive phenotyping (pleiotropy).

10.30 - 11.30

Outputs from the Harwell Ageing Screen

12.00 - 14.00

2x 1hr Small Group Tours of MLC
Phenotyping and husbandry practises.
One group will have lunch while the other group has lunch.

Session 2: Know Your Models

14.00 – 15.00

MS Models and Experiences with Drug Trials in Mice
Examples of different approaches to disease modelling and assessing outcomes of interventions. Experiences with differences in mice affecting drug trials

15.00 – 16.00

Phenotypic Outcomes of Rapamycin Treatment Throughout Life
Analysing rapamycin treatment through detailed whole life phenotyping.

16.00 – 17.00

Know Your Strains: Importance of Genetic Background
Examples of variation seen in phenotypes in different mouse strains and the importance of good practice in colony management.

19.30

Training School Dinner

Wednesday 25th November: European Infrastructures and Programmes

9.30 – 10.30 The Infrafrontier Network
10.30 - 11.15 Mutant Archives
11.15 – 12.00 The International Mouse Phenotyping Consortium
12.00– 14.00 Lunch/Discussions with Trainers
14.00 – 16.30

Bioinformatics resources
Worked examples at computers of using MGI, Ensembl and other important bioinformatics resources. Mixture of 20 minute talks and working at laptops through worked examples.